Anticoccidial compositions containing quaternary 5 - ammoniummethyl - 4-amino - 2 - cycloaliphatyl-pyrimidine salts

ABSTRACT

QUATERNARY 5-AMMONIUMMETHYL-4-AMINO-2-CYCLOALIPHATYLPYRIMIDINE SALTS OF THE FORMULA   2-R,5-((+)R1-CH2-),6-NH2-PYRIMIDINE X(-)   R=CYCLOALKYL, CYCLOALKENYL, CYCLOALKYLALKYL AND CYCLOALKENYLALKYL R1$=AMMONIUM GROUP OF WHICH THE NITROGEN ATOM IS PART OF A HETEROCYCLIC RADICAL X$=ANION OF AN ACID   AND ACID ADDITION SALTS THEREOF, EXHIBIT ANTICOCCIDIAL EFFECTS.

United States Patent 3,663,695 ANTICOCCIDIAL COMPOSITIONS CONTAINING QUATERNARY AMMQNIUMMETHYL 4- ggilllt) 2 CYCLOALIPHATYL-PYRIMIDINE lRenat Herbert Mizzoni, Long Valley, and George de Stevens, Summit, N..li., assignors to Ciba Corporation, Summit, NJ.

No Drawing. Continuation-impart of application Ser. No. 620,626, Mar. 6, 1967, which is a continuation-in-part of application Ser. No. 592,314, Nov. 7, 1966, which is a continuation-iu-part of application Ser. No. 572,671, Aug. 16, 1966, which is a continuation-impart of application Ser. No. 534,666, Mar. 16, 1966, which in turn is a continuation-impart of application Ser. No. 493,233, Oct. 5, 1965. This application Apr. 25, 1969, Ser. No. 819,459

Int. Cl. A61]; 27/00 U.S. Cl. 424-249 17 Claims ABSTRACT OF THE DISCLOSURE Quaternary S-ammoniummethyl 4 amino-Z-cycloaliphatylpyrimidine salts of the formula R=cyc1oalkyl, cycloalkenyl, cycloalkylalkyl and cycloalkenylalkyl R =ammoniurn group of which the nitrogen atom is part of a heterocyclic radical X =anion of an acid and acid addition salts thereof, exhibit anticoccidial effects.

CROSS-REFERENCES TO RELATED APPLICATIONS This is a continuation-in-part of application Ser. No. 620,626, filed Mar. 6, 1967, (now US. Pat. No. 3,454,- 575, which in turn is a continuation-in-part of applica tion Ser. No. 592,314, filed Nov. 7, 1966 (now US. Pat. No. 3,385,857), which in turn is a continuation-in-part of application Ser. No. 572,671, filed Aug. 16, 1966, which in turn is a continuation-in-part of application Ser. No. 534,666, filed Mar. 16, 1966, which in turn is a continuation-in-part of application Ser. No. 493,233, filed Oct. 5, 1965, all of which are now abandoned; except those patented.

SUMMARY OF THE INVENTION The present invention concerns and has for its object the provision of new quaternary 5-ammoniurnmethyl-4- amino-Z-cycloaliphatyl-pyrimidine salts, more particularly those of Formula I l n (I) in which R is cycloalkyl, cycloalkenyl or any such radical substituted by lower alkyl groups, halogen atoms and/or etherified hydroxy or mercapto groups, Y is a direct bond or lower alkylene, R is an ammonium group of which the nitrogen atom is part of a 5- or 6-ring-membered monocyclic heterocyclic radical or an 8- to IO-ring-rnembered bicyclic heterocyclic radical both containing up to 3 hetero atoms and at least one double bond extending from the ammonium nitrogen, and X is the anion of an acid, or acid addition salts thereof, corresponding veterinary compositions, e.g. feedstuifs and feedstutt additives, as well as methods for the preparation of these products. Said compositions, feedstulfs or drinking water contain- 5 ing the compounds of the invention alone, or in combination with other therapeutically valuable agents, are useful in the control of coccidiosis, which is one of the most important goals in the poultry raising industry.

DESCRIPTION OF THE PREFERRED EMBODIMENTS A cycloalkyl or cycloalkyl-lower alkyl radical representing RY, more particularly contains 3 to 8 ring-carbon atoms and preferably stands for cycloalkyl, (lower alkyl)- cycloalkyl, cycloalkyl-lower alkyl or (lower alkyl)-cyclo alkyl-lower alkyl with 3 to 6 ring-carbon'atoms and up to 4 lower alkyl groups. These (referred to above and hereinafter) contain up to 7, preferably up to 4 carbon atoms. Such radicals are exemplified by cyclopropyl, Z-methylcyclopropyl, 2,2- or 2,3-dimethyl-cyclopropyl, 2,2,3,3-tetramethyl-cyclopropyl, 2-ethyl-cyclopropy1, cyclobutyl, 2,2,3-trimethyl-cyclobutyl, 3-ethyl-cyclobutyl, cyclopentyl, 2- or 3-methyl-cyclopentyl, 2,5- or 3,4-dimethyl-cyclopentyl, cyclohexyl, 2-, 3- or 4-methy1-cyclohexyl, 2,3-, 2,4- or 3,5-dimethyl-cyclohexyl, 2,4,6-trimethyl-cyclohexyl, cycloheptyl or cyclooctyl or the group R -alkin which the lower alkylene portion alk separating the cycloalkyl group R (e.g. one of the above defined radicals) from the 2- position of the pyrimidine nucleus, preferably has 1 to 4 carbon atoms and especially represents methylene, but also 1,1- or 1,2-ethylene, 1,1-, 1,2-, 2,2- or 1,3-propylene, Z-methyl 1,3 propylene, 1,1-, 1,2-, 2,2-, 1,3- or 1,4- butylene. A cycloalkenyl or cycloalkenyl-lower alkyl group RY has at most 2 double bonds and, more particularly, contains 3 to 8 ring-carbon atoms. It preferably stands for cycloalkenyl, (lower alkyl)-cycloalkenyl, cycloalkenyl-lower alkyl or (lower alkyl)-cycloalkenyl-lower alkyl with 5 to 6 ring-carbon atoms, such as 2-cyclopropenyl, 1- or 2-cyclopentenyl, 2,4cyclopentadienyl, 2- or 3-methyl-2-cyclopentenyl, 4,5-dimethyl-2-cyclopentenyl, 1-, 2- or 3-cyclohexenyl, 2,5-cyclohexadienyl, 2-, 3- or 4-methyl-1- or 2-cyclohexenyl, 2,4- or 3,5- dimethyl-lor Z-cyclohexenyl, 2,4,6-trhnethyl-2,5-cyclohexadienyl 1-, 2- or 3-cycloheptenyl, 2,6-cycloheptadienyl or Z-cyclooctenyl or the group R -alk-, in which alk has the above given meaning and R is one of the above defined cycloalkenyl radicals. The above cycloaliphatic radicals may additionally be substituted, preferably in the ring, but also in the alk chain, by one or more than one halogen atoms, such as fluoro or chloro, or etherified hydroxy or mercapto groups, such as lower alkoxy or lower alkylmeercapto, e.g. methoxy, ethoxy, nor i-propoxy or -butoxy; methylor ethylrnercapto.

The ammonium group R represents especially a pyridinium, but also a quinolinium, isoquinolinium, pyridazinium, pyrirnidinium, pyrazininm, quinazolinium, phthalazinium, 1,5-, 1,6-, 1,7- or 1,8-naphthyridinium, N-lower alky1-N-pyrazolium, N-lower alkyl-N-imidazolium, thiazolinrn, oxazolium, 1,3,5-triazinium, l-lower alkyl 1H pyrrolo[3,2-b]pyridinium, 6-lower alkyl-6H- pyrrolo[3,4-b]pyridinium, thieno[3,2-b]pyridinium, thieno[2,3 b] pyridinium, pyrido [3,2 b]pyrimidinium or pyrido[2,3-b]pyrazinium radical, or a partially hydrogenated derivative thereof, such as a pyrazolinium, N-lower alkyl-N-pyrazolinum, imidazolinium, N-lower alkyl-N'- imidazolinium, thiazolinium or oxazolinium radical. It is unsubstituted or substtiuted by one or more than one of the same or of different substituents, for example, lower alkyl groups, such as those mentioned above, free or functionally converted hydroxy or mercapto groups, such as lower alkoxy or alkylmercapto, e.g. that mentioned above, halogen, e.g. fluoro, chloro or bromo, trifluoromethyl or amino, especially di-lower alkylamino, e.g. dimethylamino or diethylamino.

The anion X, as well as the acid addition salts mentioned in the beginning, are preferably derived from therapeutically useful inorganic or organic acids, especially hydrohalic acids, e.g. hydrochloric or hydrobromic acid, but also, for example, sulfuric, phosphoric, nitric or perchloric acid, aliphatic, alicyclic, aromatic or heterocyclic carboxylic or sulfonic acids, such as formic, acetic, propionic, succinic, glycollic, lactic, malic, tartaric, citric, ascorbic, maleic, hydroxymaleic, pyroracemic, phenylacetic, benzoic, p-aminobenzoic, anthranilic, p-hydroxybenzoic, salicylic, p-aminosalicylic, embonic, methanesulfonic, ethanesulfonic, hydroxyethanesulfonic, ethylenesulfonic, halobenzenesulfonic, toluenesulfonic, naphthalenesulfonic or sulfanilic acid, methionine, tryptophane, lysine or arginine.

The compounds of this invention possess valuable pharmacological properties. For example, they exhibit antiprotozoal activity, especially against parasites causing coccidiosis, such as Eimeria tenella, acervulina, adenoides, agridis, brunetti, hagani, maxima and necatrix. This can be demonstrated, for example, by the curative effect of a feed, containing about 0.0001 to about 0.02% of the compounds of this invention, given to chickens one or two days prior till 8 days after their inoculation with sporuulated oocysts of the above listed Eimeria species. The curative effect can also be demonstrated at doses between about 0.001 to 0.02% applied after inoculation with said oocysts. Besides the above mentioned utility, the compounds of this invention are also useful intermediates in the manufacture of other valuable products, particularly of pharmacologically active compounds.

Particularly useful are compounds of the formula in which R, stands for cycloalkyl, (lower alkyl)-cycloalkyl, (lower alkoxy)-cycloalkyl or (halo)-cycloalkyl with 3 to 6 ring-carbon atoms, 11 for the integer or that from 1 to 4, R for pyridinium, quinolinium, isoquinolinium, pyridazinium, pyrimidinium, pyrazinium, thiazolium, thiazolinium, oxazolium, oxazolinium, imidazolium imidazolinium or such radical substituted by up to 2 lower alkyl groups, and X for the anion of an acid, and acid addition salts thereof.

Of special value or compounds of the formula in which p stands 'for an integer from 2 to 5, n for the integer 0 or that from 1 to 2, m for an integer from 1 to 4, q for an integer from 0 to 2 and X for the anion of an acid, and acid addition salts thereof.

Especially mentioned are the 5 (2,4-dimethyl-pyridinium)-methyl-4-amino-2-cyclopropyl-, cyclopentyl-, cyclopropylmethyl, or cyclobutyl-methyl-pyrimidine chloride hydrochloride and the 5-(2-methyl-pyridinium)- methyl-4-amino-2-cyclopropy1-, cyclopentyl-, cyclopropylmethylor cyclobutylmethyl-pyrimidine chloride hydrochloride which, when given with the feed to Eimeria infected chicken in an amount between about 0.0001 to 0.02%, show an outstanding curative effect.

The compounds of the invention are prepared by methods in themselves known. Advantageously they are obtained by reacting a reactive ester or ether of a S-hydroxymethyl-4-amino-2-cycloaliphatyl-pyrimidine with a corresponding monoor bicyclic heterocyclic compound containing at least one ring-nitrogen atom and double bond, and/or converting a quaternary S-ammoniummethyl 4 amino 2 cycloaliphatyl-pyrimidine base or pseudobase into its quaternary salt and/or, if desired, converting a resulting free 4-amino-compound into its acid addition salt or converting a resulting acid addition salt into a free 4-amino-compound or into another salt and/ or converting a resulting quaternary salt into another quaternary salt.

A reactive ester of said S-hydroxymethyl compound is, for example, that of a strong inorganic or organic acid, such as a hydrohalic, sulfuric, sulfonic or carbamic acid, e.g. hydrochloric, hydrobromic, sulfuric or lower alkyl sulfuric acid, a lower alkane or benzene sulfonic acid, e.g. methane, ethane, benzene or p-toluene sulfonic acid, or unsubstituted or N-substituted carbamic acid, e.g. N,N-dimethylor N-phenyl-carbamic acid. An ether of said 5- hydroxymethyl compound is preferably a lower alkyl or phenyl-lower alkyl ether, e.g. the methyl, ethyl, isopropyl or benzyl ether. The quaternary base of pseudobase may contain hydroxy as an anion or as substituent of the heterocyclic ring containing the ammonium nitrogen. Advantageously the esters of the S-hydroxymethyl compound are reacted in the form of their acid addition salts with the free heterocyclic compound, whereas the corresponding ethers are reacted in the free form with an acid addition salt of the heterocyclic reagent.

The compounds of the invention are obtained in the free form, i.e. that having a free 4-amino group, or in the form of their acid addition salts, depending on the conditions under which the process is carried out; these salts are also included in the present invention. Acid addition salts that are obtained can be converted into the free compounds in known manner, for example, with weak alkalies, e.g. alkali metal carbonates or bicarbonates, or into other salts, for example with ion exchangers. Free compounds that are obtained, as well as the quaternary bases or pseudobases, can be converted into acid addition salts or quaternary salts respectively, by reacting them with inorganic or organic acids, especially those that are suitable for the formation of therapeutically useful salts, for example those listed for the anion X The trans-quarterni- Zation is carried out in the usual manner, advantageously with an excess of the corresponding heterocyclic base.

The above reactions are carried out according to standard methods, in the presence or absence of diluents, preferably such as are inert to the reagents and are solvents thereof, of catalysts, condensing agents and/or inert atmospheres, at low temperatures, room temperature or advantageously elevated temperatures, at atmospheric or superatmospheric pressure.

The invention further includes any variant of the present process in which an intermediate product obtainable at any stage of the process is used as starting material and any remaining steps are carried out, or the process is discontinued at any stage thereof, or in which the starting materials are formed under the reaction conditions, or in which the reaction components are used in the form of their salts. For example, the reactive ester of the 5-hydroxymethyl compound, e.g. a sulfonic acid ester, is advantageously formed under the reaction conditions, i.e. in the presence of the heterocyclic compound. Mainly those starting materials should be used in the reactions of the invention that lead to the formation of those compounds indicated above as being especially valuable.

The starting materials are known, or if they are new, may be prepared by methods in themselves known. Thus, for example, the 5-hydroxymethyl-4-amino-2-cycloaliphatyl-pyrimidines may be prepared by reacting a cycl0 aliphatic-lower alkanoic acid amidine or imido ester with a lower alkoxy-methylene malodinitrile, reducing the resulting 5-cyano-4-amino-2-cycloaliphatyl-pyrimidine to the corresponding S-amino-methyl compound, for example with catalytically activated hydrogen, and converting it into the corresponding S-hydroxy-methyl compound, for example by the action of nitrous acid. Said alcohol can be reactively esterified or etherified according to known methods, for example with a thionylhalide or phosphorus halide, a sulfuric or sulfonic acid halide, e.g. sulfuryl, tosyl or brosyl chloride. A -halomethyl compound obtained may then be reacted with an anhydrous alcohol or mercaptan in order to obtain the desired ethers. The latter may also be prepared by reacting an acid addition salt of a cycloaliphatic-lower alkanoic acid amidine with an a-alkoxymethylene-B-alkoxy-propionitrile. The quaternary bases or pseudobases may be obtained by reduction of the corresponding oxo-compounds, i.e. the cyclic amides, for example with the use of complex light metal hydrides, such as lithium aluminum hydride or sodium borohydride.

The compounds of the invention can be used, for example in the form of veterinary compositions, poultry feedstuffs or additives to feedstufrs, which are a further object of the present invention. The former contain said compounds in conjunction or admixture with inorganic or organic, solid or liquid pharmaceutical excipients suitable especially for enteral administration. Suitable excipicuts are substances that do not react with the compounds of the invention, for example water, gelatine, gums, sugars, e.g. lactose, glucose or sucrose, starches, e.g. corn starch or arrowroot, stearic acid or salts thereof, e.g. magnesium or calcium stearate, talc, alcohols, e.g. stearyl or benzyl alcohol, propylene glycol or polyalkylene glycols, alginic acid and other known medicinal excipients. The compositions may be, for example, tablets or pills, e.g. micropills, or in liquid form as solutions, suspensions or emulsions. They may be sterilized and/or contain adjuvants such as preserving, stabilizing, wetting or emulsifying agents, solution promoters, salts for regulating the osmotic pressure or buffers. They are prepared by conventional methods and contain about 0.1 to 75% more particularly 1 to 50%, of the active ingredient.

The feedstutis and additives for feedstuffs or for the drinking water contain the compounds of the invention together with conventional extenders, diluents and/or nutrients, such as sucrose, glucose, molasses, fermentation residues, cornmeal, ground and rolled oats, wheat shorts and middlings, meat scrap, oil cake, soybean and fish meal, alfalfa, clover, grass clippings and the like, mineral supplements, such as bone meal, calcium carbonate, iodized salt and the like, vitamins, such as vitamins A, B, C and D and other suitable substances, such as preservants, e.g. benzoic acid. They contain the compounds of the invention in an amount ranging between about 0.0001 and 0.1%, preferably between about 0.001 and 0.02%, whereas the additives may contain the pure substances, 'when used, for example, for the drinking water, but usually contain between about 1 and 50% thereof. The amount of the compounds of the invention administered via the veterinary compositions or the drinking water corresponds to that given with the medicated feedstuffs shown above. The veterinary compositions, feedstulfs and additives may contain other therapeutically valuable substances, for example, sulfonamides, especially N'-(6-chloro-2-pyrazinyl -su1fanilamide, or N'- (2-quinoxalinyl -sulfanilamide, but also N'- 2,6-dimethoxy-4-pyrimidyl -sulfanilamide, N- (S-ethyl-1,3,4-thiadiazol-2-yl) -sulfanilarnide, N-(5-methyl-3-isoxazolyl)-sulfanila1nide, N-(6-methoxy-3-pyridazinyl)sulfanilarnide and the N-acetyl derivative thereof,

N'- 4-methyl-2-pyrimidiny-l -sulfanilamide, N'-(2,6-dimethyl-4-pyrimidinyl)-sulfanilamide, N'-(5-methyl-1,3,4-thiadiazol-2-yl)-sulfanilamide, N'-(6-chloro-3-pyridazinyl)-sulfanilamide and the sodium salt thereof,

N'-(2-phenyl-3-pyrazolyl)-sulfanilamide, N'-(2-phenyl-5-methyl-3-pyrazolyl)-sulfanilamitle and the like. Sulfonamides of the type mentioned above can be used in approximately one fifth to one half of the amount effective as an antibacterial. In addition, the compositions, feedstuffs and additives of the invention may contain antibiotics, e.g. penicillin, streptomycin, aureomycin, terramycin, tetracyclin and the like, antiparasitic agents, e.g. methyl-4-acetamino-Z-ethoxy-benzoate, 2- amino-S-nitro-thiazole, l-(5-nitro-thiazolyl-2)-2-oxo-tetrahydroimidazole, 6,7-dialkoxyor cycloaliphatoxy-4-hydroxy-3-quinolinecarboxylic acids or their lower alkyl esters and/or tranquilizers, such as reserpine, methyl 18- epi-O-methyl-reserpate, meprobamate and the like.

The following examples illustrate the invention; temperatures are given in degrees centigrade and all parts wherever given are parts by weight. The Amberlite IRA- 400 resin, to which reference is made below, is a strong basic quaternary ammonium ion exchange resin of the type covered in US Pat. No. 2,591,573.

EXAMPLE 1 2.2 g. 5-hydroxymethyl-4-amino-2-cyclopropylmethylpyrimidine are dissolved in 15 ml. of freshly distilled 2,4- lutidine, and to this solution 2.24 g. p-toluenesulfonyl chloride are added. The mixture is allowed to stand at room temperature for 4 days. It is then diluted with diethyl ether to yield a precipitate of which the supernatant solution is decanted. The residue is dissolved in isopropanol and the product 4 times precipitated with diethyl ether until the odor of 2,41utidine disappears. The crystalline 5- (2,4-dimethyl-pyridinium) -methyl-4-amino-Z-cyclopropylmethyl-pyrimidine salt is dissolved in ml. Water and the solution is passed through an Amberlite IRA-400 resin column present in the chloride form. The aqueous eluate is concentrated in vacuo and the residue recrystallized from methanol-isopropanol to yield the 5-(2,4-dimethyl pyridinium) methyl 4 amino-Z-cyclopropylmethyl-pyrimidine chloride hydrochloride of the formula The starting material is prepared as follows:

104 g. cyclopropyl-acetonitrile are dissolved in 75 ml. anhydrous ethanol and 300 ml. diethyl ether, and through the solution anhydrous hydrogen chloride is bubbled until the uptake of 46.0 g. thereof is noted. The solid formed is filtered off after cooling and washed with anhydrous diethyl ether. The so-obtained cyclopropyl-acetic acid imino-ethylester hydrochloride is suspended in 75 ml. anhydrous ethanol and the mixture treated with ml. 7.2 N ethanolic ammonia. After stirring at room temperature for 7 hours, the mixture is maintained at this temperature overnight. It is filtered, the filtrate concentrated in vacuo and the residual white crystals filtered off to yield the cyclopropyl-acetic acid amidine hydrochloride.

The solution of 118 g. thereof in 800 ml. anhydrous ethanol is added to a solution of sodium ethylate prepared from 19.9 g. sodium and 850 ml. anhydrous ethanol, whereby the temperature is maintained at 5. The separated salt is removed by filtration and the filtrate is added dropwise within 1 hour to a stirred solution of 112.5 g. ethoxymethylidene-malodinitrile in 900 ml. anhydrous ethanol at -5 and stirring is continued for additional 2% hours. The product is filtered, washed with ethanol and dried in vacuo at 50 to yield the 5-cyano-4-amino-2- cyclopropylmethyl-pyrimidine.

The suspension of 18.2 g. thereof and 2.0 g. 10% palladium-charcoal in 200 ml. glacial acetic acid is saturated with anhydrous hydrogen chloride and hydrogenated at 40 p.s.i.-initial pressure. Three such runs are combined, diluted with water to dissolve separated salts and filtered to remove the catalyst. The filtrate is evaporated in vacuo, to the residue water is added and the distillation is repeated 3 times to remove remaining acetic acid. Finally anhydrous ethanol is added to the residue together with 10 ml. 4 N-ethanolic hydrochloric acid and the distillation is repeated to remove traces of water. The residue is finally boiled with isopropanol and filtered to yield the aminomethyl-4-amino-2-cyclopropylmethyl-pyrimidine dihydrochloride.

To a solution of 26.6 g. thereof in 400 ml. water the solution of 7.5 g. sodium nitrite in 400 ml. water is added over a 3 hour period whereby the temperature is maintained at 50-55. The homogeneous solution is stirred for 17 hours at said temperature, then concentrated by distillation in 'vacuo and finally adjusted to a pH about 7.5-8 with saturated sodium carbonate solution. The yellow residue extracted with 4 portions of boiling 90% aqueous acetone, the solution concentrated to a small volume and remaining water is removed azeotropically with ethanol. The solution is filtered, the filtrate acidified to a pH of about 4 with ethanolic hydrochloric acid and then diluted with diethyl ether. After standing for a short time, the precipitated yellow solid is filtered, ground in a mortar with diethyl ether, filtered and vacuum dried at 50 to yield the S-hydroxymethyl-4-amino-2- cyclopropylmethyl-pyrimidine hydrochloride.

1The hydrochloride obtained is dissolved in the minimum amount of water and solid potassium carbonate is added while cooling and stirring until an oily layer separates. It is extracted with n-butanol until the last extract is virtually colorless. The combined extracts are dried over potassium carbonate, filtered and evaporated in vacuo. The residue is triturated with diethyl ether, filtered, washed with ether and dried in vacuo at 50 to yield the 5-hydroxymethyl-4-amino-2-cyclopropylmethylpyrimidine.

EXAMPLE 2 19.0 g. S-hydroxymethyl-4-amino-2-cyclopropylmethylpyrimidine are dissolved in 100 ml. 2,4-lutidine with warming. Hereupon 20.4 g. p-toluenesulfonyl chloride are added portionwise while cooling, the solution is filtered and allowed to stand at 20 for 18 hours and at room temperature for 5 /2 days. It is then diluted with diethyl ether, the gummy solid triturated with diethyl ether and recrystallized from isopropanol-diethyl ether to yield the 5-(2,4-dimethyl-pyridinium)-methyl-4-amino 2 cyclopropylmethyl-pyrimidine salt melting at 238-239".

40.3 g. thereof are dissolved in 300 ml. water containing 5 ml. 0.006 N hydrochloric acid, the solution is treated with charcoal, filtered and the filtrate passed through a column of Amberlite IRA-400 resin column in the chloride form. The column is washed with 500 ml. water, the combined eluates treated again with charcoal, filtered and evaporated in vacuo. To the residue isopropanol is added, which is distilled off removing the residual water azeotropically. The dried residue is suspended in acetone, the suspension filtered, the filter cake washed with acetone and recrystallized from methanol-isopropanol (1:1), to yield the 5-(2,4-dimethyl-pyridinium)- methyl-4-amino-Z-cyclopropylmethyl-pyrimidine chloride hydrochloride, melting at 250.5 to 251 with decomposition; it is identical with the product obtained according to Example 1.

The starting material is prepared as follows: To the suspension of 41.1 g. lithium aluminum hydride in 300 ml. anhydrous diethyl ether, the solution of 100 g. cyclopropane carboxylic acid in 300 ml. diethyl ether is added slowly while stirring. The rate of addition is adjusted to maintain a gentle reflux of the solvent, and stirring and refluxing is continued for 8 hours, Hereupon the mixture is diluted with 500 ml. diethyl ether and 130 ml. 15% aqueous sodium hydroxide are stirred in while chilling. The mixture is filtered, the residue washed with diethyl ether, the filtrate dried, evaporated, the residue distilled and the fraction boiling at 122-126 collected. It represents the cyclopropyl-methanol having an n =l.4299.

89.1 g. thereof are dissolved in 350 ml. anhydrous diethyl ether, the solution cooled to 70 and 40.8 g. phosphorus tribromide are added dropwise with stirring during /2 hour. The reaction mixture is then allowed to warm up gradually to room temperature overnight and is then treated with 12 ml. water. The organic layer is separated, washed with saturated aqueous sodium carbonate and with water, dried, evaporated and the residue distilled to yield the cyclopropylmethyl bromide boiling at l02-1l0, n =l.4752.

126.5 g. thereof are added to the stirred suspension of 51.9 g. sodium cyanide in 377 ml. dimethylsulfoxide while maintaining the temperature between and Hereupon the reaction mixture is kept at for 2 hours, then cooled, diluted with 500 ml. water and extracted with diethyl ether. The extract is washed with water, 25 ml. *6 IN hydrochloric acid, dried and evaporated. The residue is distilled and the fraction boiling at 142l47 collected; it represents the cyclopropylacetonitrile, 11 1.4218.

The solution of 101 g. thereof in 61.4 g. anhydrous ethanol is gassed with hydrogen chloride until 48.3 g. thereof are consumed. After standing overnight at 20 the precipitate formed is filtered off, Washed with diethyl ether and dried under reduced pressure, to yield the hygroscopic cyclopropylacetimidic acid ethyl ester hydrochloride.

To the solution of 173.1 g. thereof in ml. anhydrous ethanol, ml. 6.3 N ethanolic ammonia are added rapidly while stirring and cooling. The reaction mixture is allowed to stand at room temperature overnight, whereupon it is filtered, the filtrate concentrated to /2 of its volume and the concentrate chilled until crystallization occurs by scratching. The cyclopropyl-acetamidine hydrochloride formed is filtered 01f, washed with anhydrous diethyl ether and dried under reduced pressure.

The solution of 136.6 g. thereof in 700 ml. anhydrous ethanol is added to the solution, prepared from 23.6 g. sodium and 950 ml. anhydrous ethanol, at 15 during A: hour while stirring. Upon addition of filter cel the mixture is filtered through a sintered glass funnel and the filtrate is added to the solution of 130 g. ethoxymethylidene-malodinitrile in 130 ml. anhydrous ethanol during 1% hours while chilling to 5 to l0. The mixture is stirred for 3 hours at this temperature, then filtered, the residue washed with cold anhydrous ethanol and dried in vacuo at 50, to yield the 5-cyano-4-amino-2-cyclopropylmethyl-pyrimidine melting at 181-1825 The suspension of 17.4 g. thereof, 2.5 g. 10% palladium charcoal and 200 ml. glacial acetic acid is saturated with anhydrous hydrogen chloride and hydrogenated at p.s.i. initial pressure. The theoretical hydrogen uptake is noted after about 6 hours. The mixture is diluted with water, filtered and the filtrate evaporated in vacuo. To the residue isopropanol is added and distilled off, in order to remove water and acetic acid. Finally the residue is heated on a steam bath under vacuum for 1 hour and recrystallized from isopropanol-methanol to yield the S-aminomethyl 4 amino-2-cyelopropylmethylpyrimidine dihydrochloride melting at 228.5 to 229.

The solution of 75.8 g. thereof in 1.1 liter water is heated to 55 with stirring and the solution of 23.0 g. sodium nitrite' in 1 liter water is added dropwise during 2% hours while stirring. The mixture is kept at 54 for 6 hours and overnight at room temperature. Hereupon it is treated with charcoal, filtered with the aid of filter cel and the filtrate concentrated to 500 ml. in vacuo. The concentrate is cooled to 5 and neutralized with saturated sodium carbonate solution to a pH 7.58.0. It is extracted with n-butanol, the extract dried and concentrated in vacuo until crystallization occurs. After addition of diethyl ether the mixture is filtered and the residue washed with diethyl ether to yield the -hydroxymethyl-4-amino- 2-cyclopropylmethyl-pyrimidine melting at 143-146.

EXAMPLE 3 18.5 g. 5-hydroxymethyl-4-amino-2-cyclopropylmethylpyrimidine are dissolved in 100 ml. 2-picoline and to the solution 19.9 g. p-toluenesulfonyl chloride are added. The mixture is worked up as described in Example 1 to yield the S-(Z-methyl-pyridinium)-methyl-4-amino 2 cyclopropylmethyl-pyrimidine chloride hydrochloride of the formula om- N 201 0152 I I CHCHzt H N CH2 melting at 225.5 to 226.5".

EXAMPLE 4 To the solution of 35.0 g. 5-hydroxymethyl-4-amino-2- cyclobutylmethyl-pyrimidine in 250 ml. 2,4-lutidine, 34.7 g. p-toluenesulfonyl chloride are added portionwise with stirring at room temperature. The mixture is then refrigerated at 15 for 2 days and allowed to stand at room temperature for 4 days. The precipitate formed is filtered off, washed with acetone and recrystallized twice from anhydrous ethanol to yield the 5-(2,4-dimethylpyridinium) methyl 4 amino 2 cyclobutylmethylpvrimidine chloride hydrochloride of the formula e CHg-N OH3. 201

/\ at I N melting at 240-242.

The starting material is prepared as follows: The solution of 50.0 g. cyclobutylcarboxylic acid in 500 ml. diethyl ether is added to the mixture of 23.6 g. lithium aluminum hydride and 500 ml. diethyl ether while stirring and refluxing. The mixture is refluxed and stirred for 3 hours, then cooled in an ice bath and 71.6 ml. 15% aqueous sodium hydroxide are added under nitrogen. The precipitate formed is filtered off, washed with diethyl ether and the filtrate evaporated. The residue is distilled and the fraction boiling at 140-143 collected; it represents the cyclobutylmethanol.

36.2 g. thereof are dissolved in 250 ml. anhydrous diethyl ether, the solution chilled to 70, and combined with the solution of 14.8 g. phosphorus tribromide in 20 ml. diethyl ether while maintaining the temperature at -65 to 60. The mixture is stirred for 2 hours and gradually warmed up to room temperature. Hereupon 10 ml. water are added and the organic layer separated. It is washed twice with saturated aqueous sodium carbonate and once with water, dried and evaporated. The residue is distilled and the fraction boiling at 134-136 collected; it represents the cyclobutylmethyl bromide.

28.5 g. thereof are added dropwise to the stirred mixture of 10.5 g. sodium cyanide and 76.4 g. dimethylsulfoxide at 50 within 10 minutes and the resulting mixture is kept for 2 hours at 70. It is then cooled, 150 ml. water are added and extracted with diethyl ether. The extract is washed with cold 6 N hydrochloric acid and water, dried, filtered and evaporated. The residue is distilled and the fraction boiling at 78/38 mm. Hg collected; it represents the cyclobutyl-acetonitrile.

To the solution of 11.6 g. thereof in 5.6 g. anhydrous ethanol, 9.9 g. anhydrous hydrogen bromide are added at -30 to -40 and the mixture is allowed to stand overnight at The crystalline material is dried in vacuo over phosphorus pentoxide to yield the cyclobutyl-acet- 10 imidic acid ethyl ester hydrobromide melting at 79-81".

22.7 g. thereof are dissolved in 10 ml. anhydrous ethanol and the solution treated with an excess of ethanolic ammonia. The mixture is stirred overnight at room temperature, concentrated to a small volume and the precipitate formed filtered off. It is washed with diethyl ether and dried in vacuo to yield the cyclobutylacetamidine hydrobromide melting at 152-156.

The solution of 18.4 g. thereof in ml. anhydrous ethanol is added to the solution, prepared from 2.3 g. sodium and 100 ml. anhydrous ethanol, at 0 during /2 an hour. The mixture' is stirred for A of an hour in the cold, filtered and the filtrate added to the solution of 12.0 g. ethoxymethylidene-malodinitrile in ml. ethanol at 0 to -5, during /2 an hour. The mixture is stirred for 4 hours at 0, the precipitate formed filtered off, washed with a little cold anhydrous ethanol and dried in vacuo to yield the S-cyano-4-amino-Z-cyclobutylmethylpyrimidine melting at 179-181 The mixture of 18.8 g. thereof, 250 ml. methanol, 50 ml. liquid ammonia and -5 g. Raney nickel is hydrogenated at 3 atm. initial pressure until the theoretical hydrogen uptake is noted. It is then filtered, the filtrate evaporated, the residue dissolved in anhydrous ethanol and the solution acidified against Congo Red with ethanolic hydrogen chloride. The precipitate formed is filtered off and washed with anhydrous ethanol to yield the 5-aminornethyl-4- amino 2 cyclobutylmethyl-pyrimidine dihydrochloride melting at 259-262.

To the solution of 82.5 g. thereof in 1.1 liter water, the solution of 24.3 g. sodium nitrite in 1 liter water is added during 2 hours at 50-55 while stirring, and stirring is continued for 6 hours at this temperature. The mixture is filtered, the filtrate concentrated in vacuo and the concentrate made basic with cold saturated aqueous sodium carbonate. The precipitate formed is filtered off, washed with a little cold water, dried and recrystallized from ethyl acetate to yield the 5-hydroxy-methyl-4-amino- 2-cyclobutylmethyl-pyrimidine melting at 137.

EXAMPLE 5 To the solution of 9.2 g. 5 hydroxymethyl 4 amino- 2 cyclobutylmethyl pyrimidine in 75 ml. 2-picoline, 9.1 g. p-toluenesulfonyl chloride are added with stirring at room temperature. The mixture is kept at 15 for 2 days and allowed to stand at room temperature for 4 days. The precipitate formed is filtered oif, washed with acetone, dried and recrystallized from anhydrous ethanol to yield the 5 (2 methyl pyridinium) methyl 4- amino 2 cyclobutylmethyl pyrimidine chloride hydrochloride of the formula melting at 220-224".

EXAMPLE 6 2.7 g. 5 chloromethyl 4 amino 2 cyclopropylmethylpyrimidine hydrochloride are suspended in 20 ml. 2,4-lutidine and the mixture is stirred for 4 hours on the steam bath. Hereupon it is chilled, diluted with diethyl ether, the supernatant liquid decanted and the residue boiled with isopropanol. Upon chilling the suspension is filtered, the residue washed with isopropanol and recrystallized from methanol-isopropanol to yield the 5-(2,4- dimethyl pyridinium) methyl 4 amino 2 cyclopropylmethyl pyrimidine chloride hydrochloride melting at 252 with decomposition; it is identical with the product obtained according to Example 1.

The starting material is prepared as follows: To the solution of 5.8 g. 5 hydroxymethyl 4 amino 2 cyclopropylmethyl pyrimidine in 10 ml. dimethylformamide,

2.45 ml. thionyl chloride are added dropwise while stirring and keeping the temperature below 5". After stirring for 2 hours in the ice bath, the mixture is allowed to stand at for 18 hours. Upon dilution with diethyl ether, the precipitate is filtered 01f, triturated with diethyl ether and acetone and recrystallized from isopropanoldiethyl ether to yield the chloromethyl 4 amino 2 cyclopropylmethyl-pyrimidine hydrochloride melting at 191- 195 with decomposition.

EXAMPLE 7 The mixture of 6.5 g. 5 chloromethyl 4 amino- 2 cyclopropylmethyl-pyrimidine hydrochloride and 30 ml. 2,4-lutidine is heated at the steam cone for 3 hours. It is allowed to stand overnight at room temperature, then diluted with acetone and the precipitate formed filtered oil. It is washed with acetone until the odor of 2,4-lutidine disappears and recrystallized from aqueous isoproponol, to yield the 5 (2,4 dimethyl pyridinium)- methyl 4 amino 2 cyclopropylmethyl pyrimidine chloride hydrochloride melting at 252253 with decomposition; it is identical with the product shown in Example 1.

The starting material is prepared as follows: To the solution prepared from 7.6 g. sodium and 200 ml. anhydrous ethanol, 61.2 g. cyclopropyl-acetamidine hydrobromide are added while stirring at 0-10. Stirring is continued for /2 an hour and hereupon 53.0 g. a-methoxymethylidene ,8 isopropoxy proponitrile (containing about 71% of the cis-isomer) are added in one portion and the mixture is stirred for hours at room temperature. The precipitate formed is filtered off, the filtrate evaporated in vacuo and the residue dissolved in methylene chloride. The solution is washed with water, the aqueous layer extracted with methylene chloride, the combined organic solutions dried, filtered and evaporated. The residue is dried in vacuo at room temperature and recrystallized from cyclohexane to yield the 5-isopropoxymethyl 4 amino 2 cyclopropylmethyl pyrimidine melting at 107.5108.5.

10.0 g. thereof are dissolved in 50 ml. anhydrous ethanol and the solution refluxed while being gassed with anhydrous hydrogen chloride for 2 hours. The mixture is cooled, concentrated with hydrogen chloride and allowed to stand for 24 hours at room temperature. It is then filtered, the residue triturated with diethyl ether and recrystallized from acetone-diethyl ether to yield the 5- chloromethyl 4 amino 2 cyclopropylmethyl pyrimidine hydrochloride melting at 183-187".

EXAMPLE 8 2.2 g. 5 hydroxymethyl 4 amino 2 (2 chlorocyclopropyl) methyl pyrimidine are dissolved in 12 ml. 2,4-lutidine with warming. Hereupon 2.0 g. p-toluenesulfonyl chloride are added while cooling. The mixture is then filtered and the filtrate allowed to stand at 20 for 2 days and at room temperature for 4 days. It is then diluted with acetone, the precipitate formed filtered off, washed with acetone and recrystallized from isopropanoldiethyl ether to yield the 5 (2,4 dimethyl pyridinium)- methyl 4 amino 2 (2 chloro cyclopropyl)- methyl-pyridine chloride hydrochloride of the formula 12 triturated with diethyl ether. The precipitate formed is filtered off, the residue washed with diethyl ether, the filtrate evaporated in vacuo, the residue distilled and the fraction boiling at 100107/ 11 mm. Hg collected; it represents the 2,2-dichloro-cyclopropyl-acetonitrile.

15.0 g. thereof are dissolved in 60 ml. anhydrous ethanol and through the cooled solution hydrogen chloride is bubbled until 3.7 g. thereof are consumed. After standing overnight in the refrigerator the precipitate formed is filtered off, washed with diethyl ether and dried to yield the 2,Z-dichloro-cyclopropyl-acetimidic acid ethyl ester hydrochloride.

97.5 g. thereof are suspended in 75 ml. anhydrous ethanol and 100 ml. 6.3 N ethanolic ammonia are added rapidly while stirring and cooling. The reaction mixture is allowed to stand at room temperature overnight. It is filtered, the filtrate evaporated and the residue recrystallized from ethanol to yield the 2,2-dichloro-cyclopropyl-acetic acid amidine hydrochloride.

68.5 g. thereof are dissolved in 350 ml. anhydrous ethanol and the solution combined with that prepared from 2.4 g. sodium and 95 ml. anhydrous ethanol. The mixture obtained is stirred for /2 hour at room temperature, then filtered through a glass funnel and the filtrate added dropwise to the solution of 13.0 g. ethoxymethylidene-malonodinitrile in 13 m1. anhydrous ethanol while keeping the temperature below 5 The mixture is stirred for 3 hours at this temperature, then filtered, the residue washed with a little cold ethanol and dried in vacuo to yield the 5- cyano 4 amino 2-(2,2-dichloro-cyclopropyl)-methylpyrimidine.

15.0 g. thereof are hydrogenated in 100 ml. 2 N ethanolic ammonia over 15.0 g. Raney nickel for 2 days at 47 p.s.i. and room temperature. The mixture is then filtered, the filtrate evaporated and the residue acidified with ethanolic hydrochloric acid. The precipitate formed is filtered off to yield the 5-aminomethyl-4-amino-2-(2-chlorocyclopropyl)-methyl-pyrimidine dihydrochloride.

The solution of 8.6 g. thereof in 115 ml. water is heated to 55 and the solution of 2.3 g. sodium nitrite in ml. water is added drop-wise while stirring. The mixture is kept at this temperature for 6 hours and stirred overnight at room temperature. Hereupon it is treated with charcoal, filtered and the filtrate concentrated to about 50 ml. in vacuo. The concentrate is cooled, neutralized with saturated aqueous sodium carbonate and extracted with nbutanol. The extract is dried an evaporated in vacuo. The residue is triturated with diethyl ether and the precipitate formed filtered off to yield the 5-hydroxymethyl-4-amino- 2- 2-chloro-cyclopropyl -methylpyrimidine.

EXAMPLE 9 In the manner described in the previous examples, the following compounds are prepared by using the equivalent amounts of the corresponding starting materials:

5-quinoliniummethyl-4-amino-2-cyclopropylmethylpyrimidine, 5-isoquinoliniummethyl-4-amino-2-cyclopentylmethylpyrimidine, 5 -pyridaziniummethyl-4-amino-2-cyclopentylmethylpyrimidine, 5-pyrimidiniummethyl-4-amino-2-cyclohexylmethylpyrimidine, 5-pyraziniummethyl-4-amino-2-cyclopentylmethylpyrimidine, 5-thiazoliniummethyl-4-amino-2- (2-cyclopentenyl -methyl-pyrimidine, 5-oxazoliniummethyl-4-amino-2-(2-cyclopropylethyl)- pyrimidine, 5-(5,6-dimethoxy-quinolinium)-methyl-4-amino-2- cyclobutylmethyl-pyrimidine, S-quinaldiniummethyl-4-amino-2-cyclohexylmethylpyrimidine, 5-lepidiniummethyl-4-amino-2-(2-cyclopentenyl)-methylpyrimidine,

5-( l-methyl-isoquinolinium methyl-4-amino-2- (2-cyclohexenyl -methyl-pyrimidine,

5- (2, 6-dimethyl-4-methoxy-pyridinium) methyl-4-amino- 2cyclopentylmethyl-pyrimidine,

5- (2,4-dimethyl-oxazolinium) methyl-4-a-mino2-cyclopropylmethyl-pyrimidine,

5-( 3-chloro-pyridiniurn -methyl-4-amino-2-( l-cyclopropylethyl) pyrimidine,

5- 3-dimethylaminopyridinium methyl-4-amino-2- cyclopentylmethyl-pyrimidine and the 2-(2-methyl-cyclopropylmethyl)-, 2- (2,3-dimethyl-cyclopropylmethyl) 2-(2-methoxy-cyclopropylmethyl)-, 2- (2,2,3 trirnethyl-cyclobutyl -methyl-, 2-cyclopentylmethyl-, 2- (2-cyclopropyl-ethyl) and 2-(2-cyclopropenylmethyl)-4-amino-5-(2-methylor 2,4-dimethylpyridinium)methyl-pyrimidine salts, particularly the corresponding chloride hydrochlorides.

EXAMPLE 10 9.70 g. 5-hydroxymethyl-4amino-Zcyclopentyl-pyrimidine are dissolved in 50 ml. 2,4-lutidine and to the solution 9.73 g. powdered p-toluenesulfonyl chloride are added at room temperature and the mixture is allowed to stand to 2 days at 15 and at room temperature for 5 days. It is filtered, the residue washed with isopropanol and recrystallized from methanol-isopropanol to yield the 5 (2,4-dimethyl-pyridinium)methyl-4-amino-2-cyclopentylpyrimidine chloride hydrochloride of the formula l -CH N oH, 201

I 13 he melting at 254255.

The starting material is prepared as follows: To the suspension of 55 g. sodium cyanide in 265 ml. dimethylsulfoxide, 105 g. cyclopentyl chloride are added at 110- 115 while stirring. The mixture is maintained at about 125 for 3 hours, then cooled and diluted to 1 liter with water. The mixture is extracted with methylene chloride, the extract washed with 6 N hydrochloric acid and water, dried, filtered and evaporated. The residue is distilled twice and the fraction boiling at 63/ 15 mm. Hg collected; it represents the cyclopentyl cyanide.

To the mixture of 18.0 g. thereof and 9.2 g. anhydrous ethanol, 16.2 g. anhydrous hydrogen bromide are added at 20 and the mixture is allowed to stand overnight at 15. The precipitate formed is filtered off, washed with a little diethyl ether, and dried in vacuo to yield the cyclopentyl-formimidic acid ethyl ester hydrobromide melting at 82-83".

To the solution of 153.6 g. thereof in 120 ml. anhydrous ethanol, saturated ethanolic ammonia is added so that an excess of ammonia is present. The mixture is stirred for 18 hours at room temperature, concentrated in vacuo and the concentrate chilled to yield the cyclopentyl-formamidine hydrobromide melting at 148.

The solution of 103.5 g. thereof in 250 ml. anhydrous ethanol is added to the solution, prepared from 12.3 g. sodium and 500 ml. anhydrous ethanol, at O to 5 during /2 an hour. The mixture is stirred for another A of an hour, then filtered and the filtrate added to the solution of 65.4 g. ethoxyrnethylidenemalodinitrile during 1 /2 hours at to The reaction mixture is stirred for 3 hours at that temperature, then filtered and the residue washed with a little ethanol. It is dried in vacuo and recrystallized from isoproponol to yield the 5-cyano-4- amino-Z-cyclopentyl-pyrimidine melting at 161-162.

The mixture of 18.8 g. thereof, 2.0 g. palladium charcoal and 250 ml. 3 N hydrochloric acid is hydrogenated at 48 p.s.i. initial pressure until 2 mol equivalents hydrogen are consumed. In the course of the reduction, the corresponding intermediate Schiffs base is formed, which is hydrolyzed to the corresponding aldehyde, which then is reduced to the hydroxymethyl compound. The mixture is filtered, the filtrate evaporated and traces of water are removed azeotropically with isoproponol and the resultant ammonium chloride is filtered off as formed in order to eliminate bumping. The residue is recrystallized twice from isopropanol to yield the S-hydroxymethyl- 4-amino-2-cyclopentyl-pyrimidine hydrochloride melting at -111.

20.2 g. thereof are dissolved in the minimum amount of Water and the solution made basic with saturated aqueous potassium carbonate. The mixture is extracted with n-butanol to yield the corresponding base melting at 101-104".

EXAMPLE 11 2.2 g. 5 hydroxymethyl 4 amino-2-cyclopentylpyrimidine are dissolved in 12 ml. redistilled 2-picoline and to the solution 2.15 g. p-toluenesulfonyl chloride are added while stirring. The reaction mixture is allowed to stand at room temperature for 36 hours. It is then diluted with diethyl ether whereby a gummy solid separates, which becomes crystalline on scratching. The excess picoline is removed by decanting the supernatant liquid, redissolving the solid in isopropanol and reprecipitating it 4 times with diethyl ether. It is finally dissolved in 100 ml. 0.06 N hydrochloric acid and the solution is passed through Amberlite IRA 400 in the chloride form. The eluate is concentrated at the steam bath yielding the 5-(2- methyl-pyridinium) methyl 4 amino 2 cyclopentylpyrimidine chloride hydrochloride of the formula momip .201 (IJHa EXAMPLE 12 melting at 232 to 233.

The starting material is prepared as follows: To the solution of 50 g. cyclopropyl-cyanide in 44 ml. ethanol and 75 ml. toluene, cooled to 5, 60 g. anhydrous hydrogen bromide are bubbled in and the mixture is kept in the freezer overnight at +5 It is then cooled in an ice bath and 195 ml. of 10% ethanolic ammonia are added while stirring and the mixture is then stirred for 4 hours at room temperature. It is filtered cold and the residue washed with ethanol-benzene (1:1), to yield the cyclopropyl-formamidine hydrobromide melting at -128".

17.3 g. thereof are added to the stirred solution, prepared from 2.3 g. sodium and ml. ethanol, kept under nitrogen. The mixture is stirred for /2 an hour, filtered and the filtrate added during 1 hour to the solution of 12.2 g. ethoxymethylene-malodinitrile (M.P. 67-68) in 100 ml. anhydrous ethanol at room temperature while stirring. The mixture is stirred at room temperature overnight, then cooled and filtered to yield the -cyano-4- amino-Z-cyclopropyl-pyrimidine melting at 188-190.

The suspension of 18 g. thereof in 900 ml. 1.46 molar methanolic ammonia, containing 2 teaspoons of Raney nickel, is hydrogenated at 48 p.s.i. at room temperature. After 13 hours about 85% of the theoretical amount of hydrogen is absorbed. The mixture is then filtered, the filtrate evaporated and the residue dissolved in 55 ml. ethanol. The solution is combined with 90 ml. ethanolic hydrochloric acid, cooled in an ice bath and the precipitate formed filtered off to yield the S-aminomethyl 4 amino 2 cyclopropyl-pyrimidine dihydrochloride melting at 17017l.

To the solution of g. thereof in 230 ml. water, kept at 5455, 200 ml. of the aqueous solution of 4.58 g. sodium nitrite are added during 4 hours while stirring. The mixture is stirred for 2 more hours at this temperature and at room temperature overnight. It is then acidified with hydrochloric acid to a pH of about 3, charcoal is added and filtered. The filtrate is made basic with sodium carbonate and extracted with n-butanol. The extract is washed With water and evaporated, While azeotropically dried with toluene. The residue is triturated with diethyl ether and filtered off to yield the S-hydroxymethyl-4-amino-2-cyclopropyl-pyrimidine melting at 158 to 162.

The mixture of 4.77 g. thereof, 25 ml. dimethylformamide and 3.62 g. thionyl chloride is kept for 1 hour at 0 and overnight at room temperature. Hereupon diethyl ether is added, the precipitate formed filtered 01f, triturated with acetone and dried to yield the S-chloromethyl-4-amino-2-cyclopropyl-pyrimidine hydrochloride melting at 186-187.

EXAMPLE 13 The mixture of 2.63 g. 5-chloromethyl-4-amino-2-cyclopropyl-pyrimidine hydrochloride and ml. a-picoline is heated at the steam cone for 3 hours. The residue formed is ground in a mortar, filtered off, washed with diethyl ether and triturated with ml. hot isopropanol, to yield the 5 (2-methylpyridinium)-methyl4-amino-2-cyclopropyl-pyrimidine chloride hydrochloride of the formula 93% E 6 (H2 N CH2N 2C1 OH CH; N H2 melting at 227228.

EXAMPLE 14 EXAMPLE 15 In the manner described in the previous examples, the following compounds "are prepared by using the equivalent amounts of the corresponding starting materials:

5 2-methyl-S-ethyl-pyridinium -methyl-4-amino-2- cyclopentyl-pyrimidine,

5 (4-methylpyridazinium) -methyl-4-amino-2- cyclopentyl-pyrimidine,

5- (4-methyl-5-ethyl-thiazolinium)-methyl-4-amino-2- cyclopentyl-pyrimidine,

1 6 S-quinoliniummethyl-4-amino-2-cyclopropyl-pyrimidine, S-isoquinoliniummethyl-4-amino-2-cyclopentylpyrimidine,

5-pyridaziniummethyl-4-amino-2-cyclopentylpyrimidine,

5 -pyrimidiniummethyl-4-amino-2-cyclohexylpyrimidine,

5-pyraziniummethyl-4-amino-2-cyclopentyl-pyrirnidine,

5 -thiazoliniummethyl-4-amino-2- (2-cyclopenteny1) pyrimidine,

5-oxazoliniummethyl-4-amino-2-cyclopropylpyrimidine,

5 5 ,6-dimethoxy-quinolinium -methyl-4-'amino-2- cyclobutyl-pyrimidine,

5-quinaldiniummethyl-4-amino-2-cyclohexyl-pyrimidine,

5-lepidiniummethyl-4-amino-Z- (2-cyclopentenyl pyrimidine,

5- l-methyl-isoquinolinium) -methyl-4-amino-2- (2- cyclohexenyl -pyrimidine,

5- (2,6-dimethyl-4-methoxypyridinium -methyl-4- amino-Z-cyclopentyl-pyrimidine,

5- 2,4-dimethyl-oxazolinium -methyl-4-amino-2- cyclopropyl-pyrimidine,

5-( 3-chloro-pyridinium -methyl-4-amino-2-cyclopropylpyrimidine, or

5- S-dimethylamino-pyridinium) methyl-4-amino-2- cyclopentylpyrimidine and the 2-(2-methyl-cyclopropyl)-,

2-( l-methyl-cyclopropyl) 2-(2,3-dimethyl-cyclopropyl)-,

2- (2-methoxy cyclopropyl) 2-cyclobutyl-,

2- 3-cyclopentenyl and 2- (2-cyclopropenyl -4-amino-5- (Z-methylor 2,4-dimethyl-pyridinium)-methylpyrimidine salts,

particularly the chloride hydrochloridcs.

EXAMPLE 16 Additive for drinking water:

G. 5 (2,4 dimethyl pyridinium) methyl 4- amino 2 cyclopropylmethyl pyrimidine chloride hydrochloride 30.00 Tetrasodium ethylenediamine-tetracetic acid 30.00 Citric acid 70.00 Sodium citrate 50.00

Confectioners sugar 120.00

An aqueous solution containing 0.01% of the active ingredient may be prepared from said additive.

EXAMPLE 17 A poultry feed containing 0.0005% of the active ingredient is prepared as follows:

Active ingredients:

5-(2,4-dimethyl pyridinium) methyl-4amino 2- cyclopropylmethyl pyrimidine chloride hydrochloride, gram 5.0

Feed formula: Pounds Corn meal 1103.0 Soybean meal, 44% protein 660.0 Alfalfa meal 30.0 Dicalcium phosphate 40.0 Limestone meal 10.0 Salt 5.0 Fish meal, 60% protein 40.0 Stabilized fat 60.0 Dried whey 40.0 Maganese sulfate 0.5 Zinc oxide 0.3 d,l-methionine 1.5 Vitamin premix 10.0

10 lb. of the vitamin composition contain: 16,000,000 LU. Vit. A, 1,000,000 I.U. Vit. D 5,000 I.U. Vit. E acetate, 6 g. Vit. K 6 mg. Vit. B12, 3 g. riboflavin, 30 g. niacin, g. calcium pantothenate and 100 g. ethoxyquin, made up to 10 lb. with corn meal.

The 5-(2,4-dimethyl-pyridinium)-methyl 4 amino-2- cyclopropylmethyl-pyrimidine chloride hydrochloride is first premixed with about 1 kg. of the finely ground feed mixture (which is supplied as such as by the manufacturer). The premix is increased to about 25 kg. with the feed and then thoroughly mixed with the main batch in a horizontal mixer.

EXAMPLE 18 A poultry feed containing 0.005% of the active ingredient is prepared as follows:

Premix: G.

5-(2,4-dimethyl pyridinium) methyl -4- amino 2 cyclopropylmethyl pyrimidine chloride hydrochloride 5.00

Wheat standard middlings 9,995.00

The ingredients are mixed thoroughly until uniformity is obtained.

Feed formula: Grams Corn meal 1,062.875 Fat 80.000 Fish meal, 60% protein 100.000 Soybean meal, 50% protein 500.000 Corn gluten meal 100.000 Dehydrated alfalfa meal 50.000 Corn distillers solubles 40.000 Di-calcium phosphate 28.000 Calcium carbonate 20.000 Iodized salt 10.000 Vitamins A and D (1,000,000 int. units A and 250,000 D/pound) 4.000 Calcium pantothenate 0.250 Butylated hydroxytoluene 0.250 Choline chloride, 25% 2.500 Riboflavin conc. (24 g. per pound) 0.125 Vitamin B (0.02 g. per pound) 1.000 Methionine 0.500 Maganese sulfate 0.500

Total weight 2,000.000

The feed formula is prepared as follows: A portion of the corn meal is introduced into the blending machine (about half of the amount to be added), The remaining corn meal, previously blended with the pre-heated, liquified fat, is added thereto and mixing is continued until uniformity is obtained. The manganese sulfate, di-calcium phosphate, calcium carbonate and iodized salt are then added with mixing, followed by the addition of the fish, soybean, corn gluten and alfalfa meal and the corn distiller solubles. After a uniform mixture has been obtained, vitamins A and D, calcium pantothenate, choline chloride, riboflavin, vitamin B and methionine are added in that order. Mixing is continued after the addition of butylated hydroxy-toluene, and maintained until a uniform product is obtained.

The premix is added to the feed formula prepared as described above in an amount sufiicient to provide a concentration of 0.005 g. of the active ingredient per 100 g. of feed in the uniformly blended mix.

Another premix, which can be used with the above feed formula or that of Example 17, is the following:

G. (I) 5-(2-methyl pyridinium) methyl-4-amino- 2-cyclopentyl pyrimidine chloride hydrochloride 23.00 (II) Confectioners sugar 100.00 (III) Solvent extracted soybean meal 877.00

18 The ingredient I can be replaced by the same amount of the active compounds described in Examples 3, 4, 5, 10, 12 and 13.

EXAMPLE 19 A poultry feed, containing 0.003% each of two active ingredients, is prepared as follows:

Premix: G.

(I) 5-(2,3-dimethyl pyridinium) methyl- 4-amino-2-cyclopropylmethyl pyrimidine chloride hydrochloride 3.0 (II) N'(6-chloro 2 pyrazinyl)-sulfanilamide 3.0 (III) Wheat standard middlings (30-80 mesh) 9,994.0

The thoroughly mixed ingredients are added to 99 kg. of the feed formula shown in Example 17 and the whole is homogenized in a horizontal mixer.

EXAMPLE 20 A poultry feed, contaaining 0.0125 and 0.005% each of two active ingredients, is prepared as follows:

Premix:

(I) 5-(2,4-dimethyl pyridinium) methyl-4- amino 2 cyclopropylmethyl-pyrimidine chloride hydrochloride 125.0 (II) N-(6-chloro 2 pyrazinyl) sulfanilamide 50.0 (III) Confectioners sugar 500 (IV) Soybean feed, solvent extracted 275.0

The premix is prepared by triturating I and II with III and the mixture is then screened through a 30 mesh screen, U.S. standard sieve size. The screened material is then blended with IV in a mixer, the thoroughly mixed ingredients are added to 999.5 kg. of the feed formula shown in Example 17 and the whole is homogenized in a horizontal mixer.

EXAMPLE 21 A poultry feed is prepared as follows:

Premix: G.

(I) 5 (2,4-dimethyl-pyridinium)-methyl-4 amino 2-cyclopropyl-pyrimidine chloride hydrochloride 105.0 (H) N-(6-chloro-2-pyrazinyl)sulfanilamide 34.0 (III) Soybean oil 18.0

(IV) Corn gluten feed 298.0

About one third of the amount of IV is combined with I and II, mixed and then screened through a 30 mesh screen, U .8. standard sieve. The remainder of 1V is then put into a mixer, III is added and the materials mixed to form a uniform dispersion to which the screened material is added and then mixed until uniformity is obtained. The resulting mixture is then added to 910 kg. of the feed formula shown in Example 17 and the whole is homogenized in a horizontal mixer.

screen, -U.S. standard sieve size; the screened material is then blended with IV in a mixer and the thoroughly mixed ingredients are added to 9999 kg. of the feed formula shown in Example 17 and the whole is homogenized in a horizontal mixer.

The above examples may otherwise be modified so as to obtain a poultry feed containing about 0.001 to 0.02% of the quaternary component shown in Examples 1 to 15 alone or in admixture with another therapeutic agent, e.g. a sulfonamide, which may be present in an amount between about 0.001 and 0.01%, for the intended purposes. In preparing the premix materials (or feedstuff additives respectively) in the above-identified examples one ma of course, substitute an equivalent amount of other carriers or nutrients respectively, such as cottonseed meal, linseed meal, oatmeal and the like.

EXAMPLE 23 Through the stirred solution of 6.35 g. 5-isopropoxymethyl-4-amino-2-cyclohexyl-pyrimidine in 50 ml. xylene and 14.7 ml. 2,4-lutidine, hydrogen chloride is bubbled at such rate that the temperature of the mixture is kept at about 80. In the beginning a white precipitate forms, which redissolves after about 45 minutes and gassing is continued for a total of one hour. Hereupon the mixture is refluxed for 1 /2 hours whereupon 7 ml. 2,4-lutidine are added and refluxing is continued for 1 /2 hours while stirring. After cooling the organic layer is decanted off, the residue washed with benzene and taken up in 50 ml. hot acetonitrile. The solution is filtered hot and the filtrate allowed to stand overnight at room temperature. The precipitate formed is filtered off and again recrystallized from acetonitrile, to yield the 5 (2,4-dimethylpyridinium)-methyl 4 amino-2-cyclohexyl-pyrimidine chloride hydrochloride of the formula melting at 209-215".

The starting material is prepared as follows: Into the mixture of 71.2 g. cyclohexyl-cyam'de, 39.2 ml. ethanol and 75 ml. toluene cooled to l m. a total of 56 g. anhydrous hydrogen bromide is introduced at such a rate to keep the temperature between about 5 and The mixture is allowed to stand overnight at 5 and the precipitate formed is filtered off to yield the cyclohexylformimidic acid ethyl ester hydrobromide.

It is added portionwise to 200 ml. 10% ethanolic ammonia while stirring and the mixture allowed to stand overnight at room temperature. It is filtered, washed with 25 ml. ethanol and the filtrate evaporated in vacuo. The residue is triturated with hot ethyl acetate and recrystallized from acetone, to yield the cyclohexyl-formamidine hydrobromide.

24.33 g. thereof are added to the solution obtained from 2.46 g. sodium and 150 ml. isopropanol, while stirring, cooling and keeping the mixture under nitrogen. After stirring for 1 hour, the solution of 16.1 g. a-rnethoxymethylidene B-isopropoxy-propionitrile in 50 ml. isopropanol is added during one hour and the mixture is allowed to stand for two days at room temperature. It is evaporated in vacuo, the residue taken up in 2% hydrochloric acid and the solution washed with diethyl ether. It is made basic with sodium carbonate, extracted with methylene chloride, the extract dried and evaporated. The residue is taken up in 500 ml. hot hexane, the mixture filtered hot and the filtrate concentrated to about 350 ml. After cooling, the 5-isopropoxymethyl-4-amino-2- cyclohexyl-pyrimidine separates, melting at 1l8120.

EXAMPLE 24 The mixture of 1 g. 5-isopropoxymethyl-4-amino-2- cyclohexyl-pyrimidine, 2 ml. a-picoline and 10 ml. xylene is stirred at room temperature and hydrogen chloride is bubbled through at a moderate rate during one hour. Hereupon the mixture is refluxed for 3 /2 hours while stirring. After cooling, the organic phase is decanted off and the viscous gum is dissolved in the minimum amount of hot isopropanol-acetonitrile. The solution is cooled and the precipitate formed filtered oil", to yield the 5 (2 methylpyridinium-methyl-4-amino-2-cyclohexylpyrimidine chloride hydrochloride of the formula melting at 204208.

We claim:

1. A veterinary composition comprising 0.0001 to of a quaternary S-ammoniummethyl 4 amino-2-cycloaliphatyl-pyrimidine salt having the formula in which R is a member selected from the group consisting of 3 to 8 ring-membered cycloalkyl, cycloalkenyl and any such radical substituted by a member selected from the group consisting of lower alkyl, halogen, lower alkoxy and lower alkylmercapto, Y is a direct bond or lower alkylene, R is a member selected from the group consisting of pyridinium, quinolinium, isoquinolinium, pyridazinium, pyrimidinium, pyrazinium. quinazolium, phthalazinium, 1,5-naphthyridinium, 1,6-naphthyridinium, 1,7- naphthyridinium, 1,8-naphthyridinium, N-lower alkyl-N'- pyrazolium, N-lower alkyl-N'-imidazolium, thiazolium, oxazolium, 1,3,5-triazinium, l-lower alkyl-lH-pyrrolo [3,2-b1pyridinium, 6-lower alkyl-fiH-pyrrolo[3,4-b1pyridinium, thieno[3,2-b1pyridinium, thieno[2,3-b]pyridinium, pyrido[3,2-b1pyrimidinium, pyrido [2,3-b] pyrazinium, pyrazolinium, N-lower alkyl-N-pyrazolinium, imidazolinium, N-lower alkyl-N-imidazolinium, thiazolinium, oxazolinium and each of said groups substituted by a member selected from the group consisting of lower alkyl, hydroxy, lower alkoxy, mercapto, lower alkylmercapto, halogen, trifiuoromethyl and di-lower alkylamino, and X is the anion of a therapeutically useful acid, or a therapeutically useful acid addition salt thereof, together with a poultry nutrient.

2. A composition as claimed in claim 1 wherein the quaternary pyrimidine salt is that of the formula in which R, is a member selected from the group consisting of 3 to 6 ring-membered cycloalkyl, (lower alkyl)- cycloalkyl, (lower alkoxy)-cycloalkyl and (halo)-cycloalkyl, Y is a direct bond or alkylene with 1 to 4 carbon atoms, R is a member selected from the group consisting of pyridinium, quinolinium, isoquinolinium, pyridazinium, pyrimidinium, pyrazinium, thiazolium, thiazolinium, oxazolium, oxazolinium, imidazolium, imidazolinium and each of such groups substituted by lower alkyl, and X is the anion of a therapeutically useful acid, and a therapeutically useful acid addition salt thereof.

3. A composition as claimed in claim 1, wherein the quaternary pyrimidine salt is that of the formula m zm H) 4 21 in which p is an integer from 2 to 5 n is the integer 0, l or 2, m is an integer from 1 to 4, q is an integer from to 2, and X is the anion of a therapeutically useful acid, and a therapeutically useful acid addition salt thereof.

4. A composition as claimed in claim 1 wherein the quaternary pyrimidine salt is a therapeutically useful (2,4 dimethyl-pyridinium)-methyl-4-amino 2 cyclopropylmethyl-pyrimidine salt.

5. A composition as claimed in claim 1 wherein the quaternary pyrimidine salt is the 5-(2,4-dimethyl-pyridinium)methy1-4-amino 2 cyclopropylmethyl-pyrimidine chloride hydrochloride.

6. A composition as claimed in claim 1, wherein the quaternary pyrimidine salt is a therapeutically useful 5- (Z-methyl-pyridinium)-methyl 4 amino 2 cyclopropylmethyl-pyrimidine salt.

7. A composition as claimed in claim 1 wherein the quaternary pyrimidine salt is the 5 (2 methyl-pyridinium)-methyl-4-amino 2 cyclopropylmethyl-pyrimidine chloride hydrochloride.

8. A composition as claimed in claim 1 wherein the quaternary pyrimidine salt is a therapeutically useful 5- (Z-methyl-pyridinium)-methyl 4 amino-Z-cyclopropylpyrimidine salt.

9. A composition as claimed in claim 1 wherein the quaternary pyrimidine salt is the 5 (2 methyl-pyridinium)-methyl-4-amino 2 cyclopropyl-pyrimidine chloride hydrochloride.

10. A composition as claimed in claim 1 wherein the quaternary pyrimidine salt is a therapeutically useful 5- (2,4 dimethyl-pyridinium)-methyl 4 amino-Z-cyclopropyl-pyrimidine salt.

11. A composition as claimed in claim 1 wherein the 22 quaternary pyrimidine salt is the 5-(2,4-dimethyl-pyridinium) methyl-4-amino-2-cyclopropyl-pyrimidine chloride hydrochloride.

12. A composition as claimed in claim 1 comprising about 1 to 0f the quaternary pyrimidine salt.

13. A composition as claimed in claim 12 wherein the quaternary pyrimidine salt is the 5-(2,4dimethyl-pyridinium)-methyl 4 amino 2 cyclopropylmethyl-pyrimidine chloride hydrochloride.

14. A composition as claimed in claim 1 comprising about 0.0001 to 0.1% of the quaternary pyrimidine salt.

15. A composition as claimed in claim 1 comprising about 0.001 to 0.02% of 5 (2,4-dimethyl-pyridinium)- methyl 4 amino-2-cyclopropylmethyl-pyrimidine chloride hydrochloride, together with a poultry nutrient.

16. A composition as claimed in claim 12 wherein the quaternary pyrimidine salt is the 5-(2,4-dimethyl-pyridinium)-methyl 4 amino-2-cyclopropyl-pyrimidine chloride hydrochloride.

17. A composition as claimed in claim 1 comprising about 0.001 to 0.02% of 5 (2,4-dimethyl-pyridinium)- methyl-4-amino 2 cyclopropyl-pyrimidine chloride hydrochloride, together with a poultry nutrient.

References Cited UNITED STATES PATENTS 3,042,676 6/1963 Rogers et a1 424251 SAM ROSEN, Primary Examiner US. Cl. X.R. 424-25 1 

